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The Protein Mapping Group is part of a multidisciplinary team of
biochemists, microbiologists, molecular biologists, and structural
biologists within the Biosciences Division at Argonne National
Laboratory (ANL). The Group uses two-dimensional gel electrophoresis
(2DE) coupled with computerized image and data analysis to characterize
normal protein expression and to detect altered protein expression
in biological systems responding to environmental or pathological
stresses. Changes in the abundance or position of proteins in the
2DE patterns that correlate with exogenous pressures suggest the
involvement of those proteins in the biological response mechanism.
Identification of such proteins by using peptide mass mapping
provides markers of biological response as well as keys to
biochemical mechanisms related to detoxification of harmful
chemicals, cellular response to metabolic stress, and commitment
to differentiation or programmed cell death. The efforts of the
Protein Mapping Group are currently focused on analysis of proteins
from microbial systems of interest to the U.S. Department of Energy. Data from prior studies of mouse and human cell systems are also available. This website is designed to interface protein expression data with genome sequence and metabolic pathway databases in order to place the observed fluctuations in protein expression into the context of the whole cell system. When publicly available, complete genome sequences are associated with the corresponding 2DE protein patterns. This site is comprised of several subsites connected through hyperlinks. The menu tabs at the top of each page are used to navigate to the subsites of interest and each subsite has a help menu describing the specific utilities available. For information regarding the software used to develop and maintain this website, please use the "Admin" button to contact the website administrator. Additional information regarding experimental protocols, data collection and analysis, and experimental results can be found under the "Methods" menu tab or in our publications.
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PMG home
M. jannaschii proteome